- 询价
- Cell Signaling Technology
- 2541
- USA
- 2025年12月11日
- W, IF-IC
- Rabbit
- H,M,R,Mk
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Phospho-Paxillin (Tyr118) Antibody
- 抗原：
synthetic phosphopeptide corresponding to residues surrounding Tyr118 of human paxillin
- 应用范围：
W, IF-IC
- 宿主：
Rabbit
- 适应物种：
H,M,R,Mk
- 保质期：
详见说明书
- 供应商：
CST
- 级别：
详见MSDS文件
- 库存：
大量
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IF-IC | H M R Mk | Endogenous | 68 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-Paxillin (Tyr118) Antibody detects endogenous levels of paxillin only when phosphorylated at tyrosine 118. The antibody does not cross-react with other tyrosine phosphorylated proteins. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr118 of human paxillin. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of untreated and lambda-phosphatase treated COS cells, using Phospho-Paxillin (Tyr118) Antibody (left) and Paxillin Antibody #2542 (right). Western Blotting
Western blot analysis of extracts from various cell lines using Phospho-Paxillin (Tyr118) Antibody. IF-IC
Confocal immunofluorescent analysis of Hela cells -/+ lamda phosphatse labeled with Phospho-Paxillin (Tyr118) Antibody (green). Note the robust signal in the untreated cells (left) versus the lambda phosphatse treated cells (right).Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). Red = phalloidin555. |
| Background | Paxillin is a multi-domain protein that localizes primarily to focal adhesion sites to the extracellular matrix (1). Paxillin is one of the key components of integrin signaling and tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization (2). Paxillin is phosphorylated by another focal adhesion component, focal adhesion kinase (FAK), at Tyr118 (3,4). Phospho-Tyr118 may provide a docking site for recruitment of other signaling molecules to focal adhesions. It has been shown that the SH2 domain of Crk binds to the phosphorylated Tyr118 of paxillin (5).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验【求助】谁有兔抗Paxillin和鼠抗phorsphotyrosine
kelvin12 请问各位同行谁有多余的兔抗Paxillin和鼠抗phorsphotyrosine，能否相赠？谢谢！ iammonkey kelvin12战友：你知道paxillin磷酸化的阻断剂是什么吗？正做相关东西，刚接触不太明白！ 磷酸化抗体 我公司有现货，具体如下： IBN4001 e-Acetylated Lysine Antibody IBN4006 e
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
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