- 询价
- Cell Signaling Technology
- 2237
- USA
- 2025年11月05日
- W, IF-IC
- Rabbit
- H,R
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Phospho-EGF Receptor (Tyr1045) Antibody
- 抗原：
synthetic phosphopeptide corresponding to residues surrounding Tyr1045 of human EGF receptor
- 应用范围：
W, IF-IC
- 宿主：
Rabbit
- 供应商：
CST
- 保质期：
详见说明书
- 适应物种：
H,R
- 级别：
详见MSDS文件
- 库存：
大量
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
40 ul (4 western blots)/100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 40 ul (4 western blots) | 产品价格： | ￥请询价 |
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: H=Human R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IF-IC | H R | Endogenous | 175 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-EGF Receptor (Tyr1045) Antibody detects EGF receptor only when phosphorylated at tyrosine1045. The antibody may cross-react with other activated EGF receptor family members (e.g. ErbB2). |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1045 of human EGF receptor. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from A431 cells, untreated or EGF-stimulated (100 ng/ml), using Phospho-EGF Receptor (Tyr1045) Antibody (upper) or EGF Receptor (C74B9) Rabbit mAb #2646 (lower). IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing membrane and cytoplasmic localization, using Phospho-EGF Receptor (Tyr1045) Antibody. IF-IC
Confocal immunofluorescent analysis of A431 cells serum-starved (left) or EGF-treated (right) and labeled with Phospho-EGF Receptor (Tyr1045) Antibody (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). |
| Background | The epidermal growth factor (EGF) receptor is a transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling, internalization, and lysosomal degradation (1,2). Phosphorylation of EGF receptor (EGFR) at Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the active state enzyme, and providing a binding surface for substrate proteins (3,4). c-Src is involved in phosphorylation of EGFR at Tyr845 (5). The SH2 domain of PLCγ binds at phospho-Tyr992, resulting in activation of PLCγ-mediated downstream signaling (6). Phosphorylation of EGFR at Tyr1045 creates a major docking site for the adaptor protein c-Cbl, leading to receptor ubiquitination and degradation following EGFR activation (7,8). The GRB2 adaptor protein binds activated EGFR at phospho-Tyr1068 (9). A pair of phosphorylated EGFR residues (Tyr1148 and Tyr1173) provide a docking site for the Shc scaffold protein, with both sites involved in MAP kinase signaling activation (2). Phosphorylation of EGFR at specific serine and threonine residues attenuates EGFR kinase activity. EGFR carboxy-terminal residues Ser1046 and Ser1047 are phosphorylated by CaM kinase II; mutation of either of these serines results in upregulated EGFR tyrosine autophosphorylation (10).
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| Application References |
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验EGF受体是一种糖蛋白, 广泛分布于哺乳动物的上皮细胞、人的成纤维细胞、胶质细胞、角质细胞等。EGF 受体是一条含有1186个氨基酸残基的多肽链, 相对分子质量为170kDa,由三个部分组成:①很大的细胞外结构域：约621个氨基酸残基，富含半胱氨酸(51个), 并形成多对二硫键，其上结合有糖基,是EGF结合的位点。②跨膜区∶由23个氨基酸残基组成;③细胞质结构域,由542个氨基酸残基组成,含有无活性的酪氨酸激酶和几个酪氨酸磷酸化的位点。
Assays to Monitor Degradation of the EGF Receptor
to conduct receptor downregulation assays are described. Monitoring of ubiquitination and degradation of the EGFR subsequent to stimulation with the receptor ligand EGF is described. Finally, protocols to quantitatively measure degradation of the EGFR
Motility Signaled From the EGF Receptor and Related Systems
Cell motility is now recognized as central to many biological processes. Growth factors, such as those that activate the epidermal growth factor receptor (EGFR), drive biochemically and biologically distinct subsets of migration critical
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