- 询价
- Cell Signaling Technology
- 9832
- USA
- 2025年11月21日
- W, IP, IF-IC, F
- H,M,R,Hm,Mk
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
GSK-3β (3D10) Mouse mAb
- 抗原：
recombinant protein specific to the carboxy-terminus of human GSK-3β protein
- 应用范围：
W, IP, IF-IC, F
- 库存：
大量
- 供应商：
CST
- 适应物种：
H,M,R,Hm,Mk
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat Hm=Hamster Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IF-IC F | H M R Hm Mk | Endogenous | 46 | Mouse IgG2a |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | GSK-3β (3D10) Mouse mAb recognizes endogenous levels of total GSK-3β protein. This antibody does not cross-react with GSK-3α. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy-terminus of human GSK-3β protein. Western Blotting
Western blot analysis of extracts from wild-type, GSK-3α (-/-), and GSK3β (-/-) mouse embryonic fibroblasts (MEFs) and HeLa cells using GSK-3β (3D10) Mouse mAb (upper) and GSK-3α/β (D75D3) XP® Rabbit mAb #5676 (lower). (MEF wild type, GSK-3α (-/-) and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada). Flow Cytometry
Flow cytometric analysis of wild-type mouse embryonic fibroblasts (MEFs) (green) and GSK-3β (-/-) MEFs (blue) using GSK-3β (3D10) Mouse mAb. (MEF wild type and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada). IF-IC
Confocal immunofluorescent analysis of wild-type mouse embryonic fibroblasts (MEFs) (left) and GSK-3β (-/-) MEFs (right) using GSK-3β (3D10) Mouse mAb (green). Red = Propidium Iodide/RNase (fluorescent DNA dye). (MEF wild type and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada). |
| Background | Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
Staining Whole Mouse Embryos for β-Galactosidase (lacZ) Activity
Staining Whole Mouse Embryos for β-Galactosidase (lacZ ) Activity Andras Nagy , Marina Gertsenstein , Kristina Vintersten , and Richard Behringer This protocol was adapted from "Techniques for Visualizing Gene Products, Cells, Tissues
Methodologies for Staining and Visualisation of β-Galactosidase in Mouse Embryos and Tissues
This chapter provides information on β-galactosidase staining of whole mouse embryos, organs, tissue sections, and cultured cells, as well as double staining with horseradish peroxidase and use as a tool for genotyping. Using these protocols
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