- 询价
- Cell Signaling Technology
- 3137
- USA
- 2025年07月16日
- W
- Rabbit
- H,M
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- 详细信息
- 技术资料
- 抗体英文名：
Phospho-Ack1 (Tyr857/858) Antibody
- 抗原：
synthetic phosphopeptide corresponding to residues surrounding Tyr857/858 of human Ack1
- 应用范围：
W
- 宿主：
Rabbit
- 库存：
大量
- 适应物种：
H,M
- 级别：
详见MSDS文件
- 保质期：
详见说明书
- 供应商：
CST
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M | Transfected Only | 135 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-Ack1 (Tyr857/858) Antibody detects overexpressed levels of Ack1 only when phosphorylated at Tyr857/858. This antibody does not cross-react with other tyrosine-phosphorylated tyrosine kinases. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr857/858 of human Ack1. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from COS cells, untransfected or overexpressing Myc-tagged Ack1 protein, using Phospho-Ack1 (Tyr857/858) Antibody (upper and middle), and Myc-tag (71D10) Antibody #2278 (lower). The middle blot was treated with phosphatase before antibody probing. The lysate of COS cells overexpressing Myc-tagged Ack1 protein was kindly provided by Dr. Wannian Yang. |
| Background | Ack1 and Ack2 (activated cdc42-associated kinase 1 and 2) are non-receptor tyrosine kinases that consist of a tyrosine kinase core, an SH3 domain, a cdc42/Rac-binding (CRIB) domain, a Ralt homology region and a proline-rich region (1,2). Ack1 and 2 are the only two tyrosine kinases known to interact with cdc42. Both Acks are activated by growth factors including EGF and PDGF, as well as by activated integrins through cell adhesion, and may serve to link receptor tyrosine kinase or G protein-coupled receptor signaling with cdc42. Acks may regulate cell growth, morphology and motility (3,4). Recent findings indicate that Ack1 may play a role in prostate tumorigenesis, making it a potential drug target for this type of cancer (5). Phosphorylation of Ack1 on Tyr857 and Tyr858 on Ack1 was identified at Cell Signaling Technology (CST) using PhosphoScan® , CST's LC-MS/MS platform for phosphorylation site discovery as well as other publications using MS technology (5,6). Phosphorylation of Ack1 at Tyr857/858 was observed in select carcinoma cell lines and in tumors.
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| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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