Applications Key: W=Western Blotting Reactivity Key: H=Human Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
PKR (N216) Antibody detects endogenous levels of total PKR protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human PKR. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from various cell lines, using PKR (N216) Antibody.
Background
Protein kinase R (PKR) is transcriptionally induced by interferon and activated by double-stranded RNA (dsRNA). PKR inhibits translation initiation through phosphorylation of the alpha subunit of the initiation factor eIF2 (eIF2alpha) and also controls the activation of several transcription factors such as NF-kappaB, p53 and the Stats. In addition, PKR mediates apoptosis induced by many different stimuli, such as LPS, TNF-alpha, viral infection and serum starvation (1,2). Activation of PKR by dsRNA results in PKR dimerization and autophosphorylation at threonine 446 and threonine 451 in the activation loop. Substitution of threonine 451 to alanine completely inactivates PKR, while a mutant with a threonine 446 to alanine substitution was partially active (3). Recently, activation of PKR has been implicated in the pathologies of neurodegenerative diseases, including Alzheimer disease (4,5).