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文献和实验The MTT Cell Proliferation Assay
Cell number per well too high. Decrease cell density at plating.Contamination of culture with bacteria or yeast. Remedy Discard. View wells prior to addition of MTT Reagent to check for contamination. 4、Problem: Absorbance readings are too low
and then calculate cell viability and concentration. 3. Use of HepaRG™ cells 1) At day 0, 4 hours after plating a. Four hours after plating observe cell morphology under phase-contrast microscope, and when possible, take photomicrographs
CellTrace CFSE Cell Proliferation Kit
Materials Needed but Not Included: Cells of interest as a single-cell suspension PBS/0.1% BSA 5 mM stock solution of CFSE probe (see Reagent Preparation) Culture media for cells of interest Flow cytometer with 488
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