- 询价
- Cell Signaling Technology
- 3729
- USA
- 2025年07月13日
- W, IF-IC, F
- Rabbit
- H,Mk,B
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- 详细信息
- 技术资料
- 抗体英文名：
OSR1 Antibody
- 抗原：
synthetic peptide corresponding to residues at the amino terminus of human OSR1
- 应用范围：
W, IF-IC, F
- 宿主：
Rabbit
- 级别：
详见MSDS文件
- 适应物种：
H,Mk,B
- 库存：
大量
- 供应商：
CST
- 保质期：
详见说明书
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human Mk=Monkey B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IF-IC F | H Mk B | Endogenous | 60 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | OSR1 Antibody detects endogenous levels of total OSR1 protein. This antibody does not cross-react with SPAK or other members of the GCK family. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues at the amino terminus of human OSR1. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from 293, HeLa, Jurkat and COS cells using OSR1 Antibody. Flow Cytometry
Flow cytometric analysis of HeLa cells using OSR1 Antibody (blue) compared to a nonspecific negative control antibody (red). IF-IC
Confocal immunofluorescent analysis of HeLa cells using OSR1 Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
| Background | SPAK (STE20/SPS1-related Pro/Ala-rich kinase) and OSR1 (oxidative stress responsive 1) are members of the GCK family serine/threonine kinases. Overexpression and in vitro studies demonstrate that SPAK is able to activate p38 MAP kinase indicating a possible role for SPAK in the stress response (1). Yeast two-hybrid screening revealed that SPAK and OSR1 bind to Na-K-2Cl cotransporters NKCC1 and NKCC2 and K-Cl cotransporter KCC3 (2). WNK1 and WNK4 phosphorylate SPAK at Thr243/247 and Ser380 (3-5). Similarly, WNK1 and WNK4 phosphorylate OSR1 at Thr185 and Ser315 (3, 4). Phosphorylation at these sites stimulates SPAK and OSR1 activity, leading to NKCC1 phosphorylation and enhanced NKCC1 activity (3-5). SPAK is also phosphorylated at Ser311 by PKCθ in response to T cell activation. Substitution of Ser311 with Ala or specific siRNA knock-down of SPAK dramatically reduces TCR/CD28-induced AP-1 activation, suggesting SPAK is involved in T cell signaling as well (6).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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