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文献和实验Cytometry Assay to measure Internalization of GPCR's (Von Zastrow lab) Internalization of receptor is measured as the reduction of receptors on the plasma membrane surface. Surface receptors are labeled with fluoresent antibodies which is in turn measured
bovine serum (FBS). 4. GlutaMAX solution (Life Technologies). 5. 100× Pen-Strep: 100 U/μL penicillin, 100 μg/μL streptomycin. 6. OptiMEM medium (Life Technologies). 7. TrypLE® Express Enzyme (Life Technologies). 8. Transfection reagent
v468.Chapter 8 采用 b-Catenin/TCF基本转录通讯结构对b-Catenin/TCF转录进行分析
wasachieved in a 384-well plate format and it is foreseeable that thiscan be achieved in a 1,536-well format as well. The luciferaseassay reagent to be used depends on throughput of the assay. Thestandard low-throughput reagent is Promega’s Dual-Luciferase ®
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