- 询价
- Cell Signaling Technology
- 3035
- USA
- 2025年08月29日
- W, IP, ChIP
- Rabbit
- H,Mk
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
NF-κB1 p105/p50 Antibody
- 抗原：
synthetic peptide corresponding to amino acids at the amino-terminus of human NF-kappaB p105
- 应用范围：
W, IP, ChIP
- 宿主：
Rabbit
- 保质期：
详见说明书
- 适应物种：
H,Mk
- 级别：
详见MSDS文件
- 供应商：
CST
- 库存：
大量
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
40 ul (4 western blots)/100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 40 ul (4 western blots) | 产品价格： | ￥请询价 |
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation ChIP=Chromatin IP
Reactivity Key: H=Human Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP ChIP | H Mk | Endogenous | 50 Active form. 120 Precursor. | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | NF-kappaB p105/p50 Antibody detects endogenous levels of the precurser protein p105 and its cleavage product p50. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids at the amino-terminus of human NF-kappaB p105. Western Blotting
Western blot analysis of extracts from Vero cells, untreated or treated with TNF-α #2169 (20 ng/ml) for the times indicated, using Phospho-NF-κB p105 (Ser933) (18E6) Rabbit mAb #4806 (upper) and NF-κB p105/p50 Antibody #3035 (lower). Western Blotting
Western blot analysis of extracts from Hela cells, untreated or treated with TNF-alpha (12ng/ml) for the indicated amounts of time, using NF-kappaB p105/p50 Antibody. Chromatin IP
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HeLa cells treated with Human Tumor Necrosis Factor-α (hTNF-α) #8902 (30ng/ml) for 1 hour and either 20 μl of NF-κB1 p105/p50 Antibody #3035 or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by Real-Time PCR using SimpleChIP® Human IkB-alpha Promoter Primers #5552, human IAP2 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
| Background | Transcription factors of the nuclear factor κ B (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which is then translocated to the nucleus (9-11). Following IKK-mediated phosphorylation of p105 NF-κB at multiple sites (Ser921, 923, 927, and 932) on its carboxy-terminus, SCF/β-TrCP mediated processing produces the 50 kDa active form p50 (12,13).
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| Application References |
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验【进展|热点】Nature Immunology：发现三个调控NF-kB非经典信号通路的microRNA
://www.nature.com/ni/journal/v11/n9/abs/ni.1918.html 内容简介： NF-κB是一组重要的转录因子，参与了诸多免疫反应过程，其中最为人熟知的就是在天然免疫中，NF-κB是最终启动炎症因子表达的因素之一。 NF-κB家族包括5个成员，即RelA(p65)、RelB、c-Rel、p105-p50(NF-κB1)和p100-p52(NF-κB2)，两两形成同源或者异源二聚体的形式发挥作用。通常情况下，NF-κB与其抑制蛋白IκB（包括IκBα、IκBβ
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
-AT主要参与T细胞的活化。上面已经提到，胞质中无活性状态的NF-AT以磷酸化的形式存在，称为NF-ATp，钙调磷酸酶作用后使其脱磷酸化而被激活并发生转位。而钙调磷酸酶能发挥作用，是信号转导的结果。 2．NF-κB NF含义同上。κB指B细胞x链，由两个亚单位p50和p65组成，其发现和定名是因为参与B细胞活化。现知它是一种分布广泛和十分重要的转录因子。通常，NF-κB在胞质中和抑制因子LxB以复合物的形式存在。PKC可使I-κB发生磷酸化造成该复合物解离，从而解除了I-κB对NF-κB
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