- 询价
- Cell Signaling Technology
- 4914
- USA
- 2025年08月07日
- W, IP
- Rabbit
- H,Mk,B
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 技术资料
- 抗体英文名：
Hic-5 Antibody
- 抗原：
synthetic peptide corresponding to residues surrounding Ala104 of human Hic-5
- 应用范围：
W, IP
- 宿主：
Rabbit
- 保质期：
详见说明书
- 库存：
大量
- 供应商：
CST
- 适应物种：
H,Mk,B
- 级别：
详见MSDS文件
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation
Reactivity Key: H=Human Mk=Monkey B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H Mk B | Endogenous | 50 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Hic-5 Antibody detects endogenous levels of total Hic-5/ARA55 protein. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala104 of human Hic-5. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from U-2-OS, COS-7 and A-431 cells using Hic-5 Antibody. IP
Immunoprecipitation of Hic-5 from 293 cell lysates using Hic-5 Antibody or rabbit IgG control. Western blot was performed using a Hic-5 Mouse mAb. |
| Background | Hic-5 is a LIM domain family member orginally identified as a TGFbeta1 and hydrogen peroxide inducible gene, and is nearly identical to the androgen receptor co-activator ARA55 (1-3). Hic-5 is structurally related to paxillin, and both proteins are localized to focal adhesions and thought to serve as adaptor molecules, linking signals from the extracellular matrix to cytoskeletal regulation and intracelluar signaling (4,5). Like paxillin, Hic-5 contains four LD motifs and four LIM domains. Expression of Hic-5 can affect cell growth and differentiation (6-8). Increased expression of Hic-5 is observed during cellular senescence in fibroblasts, and ectopic expression in immortalized fibroblasts suppressed cell growth (8). Unlike paxillin, Hic-5 may translocate to the nucleus in response to oxidants like hydrogen peroxide (9). It has been proposed that Hic-5 serves to shuttle redox signaling from focal adhesions to the nucleus where it acts as a transcriptional co-activator for some transciption factors including, Sp1 and PPARgamma (7,9,10). Phosphorylation of Hic-5 at Tyr60 by CAKbeta and Fyn may activiate Hic-5 signaling by allowing binding to downstream SH2 domain containing proteins (11).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
技术资料暂无技术资料 索取技术资料
