- 询价
- Cell Signaling Technology
- 9587
- USA
- 2025年08月09日
- W, IP, IHC-P, IHC-F, ChIP
- Rabbit
- H,M,R,Mk,C,X,B,Dg,Pg,Hr
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- 详细信息
- 技术资料
- 抗体英文名：
β-Catenin Antibody (Carboxy-terminal Antigen)
- 抗原：
synthetic peptide corresponding to the carboxy-terminus of human β-catenin
- 应用范围：
W, IP, IHC-P, IHC-F, ChIP
- 宿主：
Rabbit
- 级别：
详见MSDS文件
- 库存：
大量
- 保质期：
详见说明书
- 适应物种：
H,M,R,Mk,C,X,B,Dg,Pg,Hr
- 供应商：
CST
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
40 ul (4 western blots)/100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 40 ul (4 western blots) | 产品价格： | ￥请询价 |
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) ChIP=Chromatin IP
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey C=Chicken X=Xenopus B=Bovine Dg=Dog Pg=Pig Hr=Horse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IHC-P IHC-F ChIP | H M R Mk (C) (X) (B) (Dg) (Pg) (Hr) | Endogenous | 92 | Rabbit |
| Protocols | |
|---|---|
| Specificity / Sensitivity | Beta-Catenin Antibody detects endogenous levels of total β-catenin protein. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminus of human β-catenin. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of total cell extracts from 293 and NIH/3T3 cells using β-Catenin Antibody (Carboxy-terminal Antigen). IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using β-Catenin Antibody (Carboxy-terminal Antigen). IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human clear cell carcinoma, using β-Catenin Antibody (Carboxy-terminal Antigen). IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using β-Catenin Antibody (Carboxy-terminal Antigen). IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded Apc (Min/+) mouse intestinal adenoma using β-Catenin Antibody (Carboxy-terminal Antigen). IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using β-Catenin Antibody (Carboxy-terminal Antigen) in the presence of control peptide (left) or beta-Catenin blocking peptide #1002 (right). IHC-F (frozen)
Immunohistochemical analysis of frozen HCC827 xenograft using beta-Catenin Antibody (Carboxy-terminal Antigen). Chromatin IP
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HCT116 cells and either 20 μl of β-Catenin Antibody (Carboxy-terminal Antigen) or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using using SimpleChIP® Human CaMK2D Intron 3 Primers #5111, human c-Myc promoter primers and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. Chromatin IP
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HCT116 cells and either 20 μl of β-Catenin Antibody (Carboxy-terminal Antigen) or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using using SimpleChIP® Human Axin2 Intron 1 Primers #8973, SimpleChIP® Human CaMK2D Intron 3 Primers #5111, human c-Myc promoter primers and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
| Background | β-catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3 (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).
|
| Application References |
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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