- 询价
- Cell Signaling Technology
- 2707
- USA
- 2025年07月09日
- 流式细胞（Flow Cyt）
- 兔
- 人
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- 文献和实验
- 技术资料
- 抗体英文名：
Zap-70 (136F12) Rabbit mAb (Alexa Fluor 647 Conjugate)
- 抗原：
/
- 应用范围：
流式细胞（Flow Cyt）
- 宿主：
兔
- 适应物种：
人
- 供应商：
CST
- 级别：
详见MSDS文件
- 抗原来源：
/
- 保质期：
详见说明书
- 库存：
大量
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (50 tests)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> /100 ul (50 tests)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a>
| 规格： | 产品价格： | ￥面议 | |
|---|---|---|---|
| 规格： | 100 ul (50 tests) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
| 规格： | 100 ul (50 tests) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
Product Pathways - Lymphocyte Signaling
Zap-70 (136F12) Rabbit mAb (Alexa Fluor® 647 Conjugate) #2707
PhosphoSitePlus ® protein, site, and accession data: ZAP70
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| F | H | Endogenous | 70 | Rabbit IgG |
Applications Key: F=Flow Cytometry
Reactivity Key: H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 2707:
- Flow
Specificity / Sensitivity
Zap-70 (136F12) Rabbit mAb (Alexa Fluor® 647 Conjugate) detects endogenous levels of total Zap-70 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the amino-terminus of human Zap-70. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions with an F/P ratio of 2-6. The Alexa Fluor® 647 dye is maximally excited by red light (e.g. 633 nm He-Ne laser). Antibody conjugates of the Alexa Fluor® 647 dye produce bright far-red-fluorescence emission, with a peak at 665 nm.
Flow Cytometry
Flow cytometric analysis of Ramos B (blue) and Jurkat T (green) cells using Zap-70 (136F12) Rabbit mAb (Alexa Fluor® 647 Conjugate).
Flow Cytometry
Two-color flow cytometric analysis of a mixed population of T and B cells (Jurkat and Ramos, respectively) using Zap-70 (136F12) Rabbit mAb (Alexa Fluor® 647 Conjugate) and a CD3 antibody. CD3 negative B cells have little or no Zap-70 staining, while CD3 positive T cells stain brightly for Zap-70 protein.
Description
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometric analysis of human cells.
Background
The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement (1). Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck (2-6). Tyrosine phosphorylation correlates with increased Zap-70 kinase activity and downstream signaling events. Expression of Zap-70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemia (7,8).
- Chu, D.H. et al. (1998) Immunol. Rev. 165, 167-180.
- Iwashima, M. et al. (1994) Science 263, 1136-1139.
- Neumeister, E.N. et al. (1995) Mol. Cell Biol. 15, 3171-3178.
- Chan, A.C. et al. (1995) EMBO J. 14, 2499-2508.
- Williams, B.L. et al. (1999) EMBO J. 18, 1832-1844.
- Di Bartolo, V. et al. (1999) J. Biol. Chem. 274, 6285-6294.
- Wiestner, A. et al. (2003) Blood 101, 4944-4951.
- Crespo, M. et al. (2003) N. Engl. J. Med. 348, 1764-1775.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
Companion Products
- 2701 Phospho-Zap-70 (Tyr319)/Syk (Tyr352) Antibody
- 2704 Phospho-Zap-70 (Tyr493) Antibody
- 2705 Zap-70 (99F2) Rabbit mAb
- 2985 Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 647 Conjugate)
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
For Research Use Only. Not For Use In Diagnostic Procedures.
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文献和实验CST 抗体 9 月特惠｜购买任意 CST 抗体，+168 元即可入手 25μl 标签 / 内参抗体
HRP、Alexa Fluor® 488、Alexa Fluor® 594 等多种标记一抗可选。 热门可选产品参考 货号 产品名称 类型 加价购价格 S0B0005-25u1 B-actin Rabbit mAb (SDT-RO15) 裸抗 168 元 S0B0004-25u1 B-tubulin Recombinant Rabbit mAb (SDT-RO14) 裸抗 168 元 S0B0383-25u1 S-RMab® Myc tag Recombinant Rabbit
Antibody250µlIF-IC HCA4410SAnti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate)Secondary Antibody250µlIF-IC F4412SAnti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate)Secondary Antibody250µlIF-IC F4413SAnti-rabbit IgG (H+L
：使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。（图 A）使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit，抗体 1:100 稀释；(图 B) 采用普通免疫组化试剂盒，抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注：使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK （Erk1/2）Rabbit mAb 对正常小鼠心脏组织进行免疫
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