- 询价
- Biotium
- 美国
- 31032
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
4°C
- 保质期：
6个月
- 英文名：
Ready-to-Use 100 BP DNA Ladder 31032
- 库存：
99
- 供应商：
Biotium
- CAS号：
1
Ready-to-Use 100 bp DNA Ladder is suitable for sizing linear double-stranded DNA fragments from 100 bp to 1500 bp. The ladder consists of 11 bands that are generated from PCR and restriction enzyme digestion of double stranded DNA. The 500 bp and 1500 bp bands contain more DNA to provide internal orientation.
- Contains 11 double-stranded DNA fragments
- Bands range from 100 bp to 1500 bp
- 500 bp and 1500 bp bands are higher concentration, for orientation
- Provided pre-diluted in 1X loading buffer, ready to load
- Ladder concentration is 20 ng/uL
Ready-to-Use Ladders are provided pre-diluted in 1X loading buffer at a concentration of 20 ng/uL. A separate vial of 6X loading buffer is provided to add to your DNA samples. The loading buffer provided contains density agents and two blue electrophoresis tracking dyes that run at approximately 1.5 kb and 200 bp in a 1% agarose gel. We also offer Ready-to-Use 1 kb DNA Ladder (31022).
To learn about our all of our products related to DNA gel staining, visit the technology page.
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文献和实验；还有一个原因是你设置的电压太高，最好是2~4v/cm，跑4～6小时比较合适（2）“我是按说明书上0。8%的琼脂糖”：凝胶浓度太低了，把凝胶浓度加大到2％，放心做，没问题的（3）“说明书上说混合温和”：那是怕你混匀太剧烈导致细胞基因组DNA断裂啊，注意很轻轻的混匀（4）“收集凋亡细胞时离心时转速”：我在实验室中是5000rpm，离心5min，我觉得足已mmyycc ：我的ladder总是只有一条带，与对照组差别不大，用的是100v,1-1.5h,不知道是不是这个原因？溴酚兰跑到什么位置合适
(2)“我是按说明书上0。8%的琼脂 糖”：凝胶浓度太低了，把凝胶浓度加大到2%，放心做，没问题的 (3)“说明书上说混合温和”：那是怕你混匀太剧烈导致细胞基因组 DNA断裂啊，注意很轻轻的混匀 (4)“收集凋亡细胞时离心时转速”：我在实验室中是5000rpm，离心5min，我觉得足已 mmyycc ：我的ladder总是只有一条带，与对照组差别不大，用的是100v,1-1.5h,不知道是不是这个原因?溴酚兰跑到什么位置合适呢? chujun_hust
EDTA) for 10 minutes at 100 V. 2) To 3 μg (3 μl) of ladder, add loading buffer (recommended final loading buffer concentration is 20 mM MOPS; pH 7.0, 6% w/v formaldehyde, 31% v/v deionized formamide, 1 mM EDTA, 0.0125% xylene cyanol
