- 询价
- Cell Signaling Technology
- 2929
- USA
- 2025年09月30日
- western blot,免疫组化（IHC）,免疫荧光（IF）,流式细胞（Flow Cyt）
- 小鼠
- 人
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 抗体英文名：
EpCAM (VU1D9) Mouse mAb
- 抗原：
/
- 应用范围：
western blot,免疫组化（IHC）,免疫荧光（IF）,流式细胞（Flow Cyt）
- 宿主：
小鼠
- 适应物种：
人
- 库存：
大量
- 抗原来源：
/
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 供应商：
CST
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a>
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
Product Pathways - Adhesion
EpCAM (VU1D9) Mouse mAb #2929
PhosphoSitePlus ® protein, site, and accession data: EPCAM
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IHC-P IF-IC F | H | Endogenous | 40 | Mouse IgG1 |
Applications Key: W=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 2929:
- Flow , IHC / Paraffin , Immunofluorescence , Western Blotting
Specificity / Sensitivity
EpCAM (VU1D9) Mouse mAb detects endogenous levels of total EpCAM protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with NCI-H69 small cell lung carcinoma cells.
Western Blotting
Western blot analysis of extracts from HT-29 and HCT-15 cells using EpCAM (VU1D9) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using EpCAM (VU1D9) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon using EpCAM (VU1D9) Mouse mAb.
Flow Cytometry
Flow cytometric analysis of unpermeabilized HT-29 cells using EpCAM (VU1D9) Mouse mAb (blue) compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent analysis of HT-29 cells using EpCAM (VU1D9) Mouse mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Background
Epithelial cell adhesion and activating molecule (EpCAM/CD326) is a transmembrane glycoprotein that mediates Ca2+ -independent, homophilic adhesions on the basolateral surface of most epithelial cells. EpCAM is not expressed in adult squamous epithelium, but it is highly expressed in adeno and squamous cell carcinomas (1). One of the first tumor-associated antigens discovered, EpCAM has long been a marker of epithelial and tumor tissue. Because of its high expression in cancer, EpCAM has become a therapeutic target for human solid tumors of epithelial lineage (reviewed in 2,3).
- Went, P.T. et al. (2004) Hum Pathol 35, 122-8.
- Baeuerle, P.A. and Gires, O. (2007) Br J Cancer 96, 417-23.
- Armstrong, A. and Eck, S.L. Cancer Biol Ther 2, 320-6.
Application References
- Luk, J.M. et al. (2011) J Biol Chem 286, 30706-13. Applications: IHC-P (paraffin)
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
Companion Products
- 7072 Phototope® -HRP Western Blot Detection System, Anti-mouse IgG, HRP-linked Antibody
- 7076 Anti-mouse IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 8112 SignalStain® Antibody Diluent
For Research Use Only. Not For Use In Diagnostic Procedures.
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- 作者
- 内容
- 询问日期
文献和实验(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
T-Cell Activation Using mAb to CD3
One of the most common ways to assess T cell activation is to measure T cell proliferation upon in vitro stimulation of T cells via antigen or agonistic antibodies to TCR. This protocol is written as a starting point for examining in vitro proliferation of mouse
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