产品封面图

NCK1 (15B9) Rabbit mAb

收藏
  • 询价
  • Cell Signaling Technology已认证
  • USA
  • 2025年08月22日
  • W, IP
  • H,M,R,Mk
avatar
品牌商
13钻石会员
  • 企业认证

    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体英文名

      NCK1 (15B9) Rabbit mAb

    • 抗原

      synthetic peptide corresponding to residues surrounding Gly300 of human NCK1

    • 应用范围

      W, IP

    • 保质期

      详见说明书

    • 级别

      详见MSDS文件

    • 供应商

      CST

    • 适应物种

      H,M,R,Mk

    • 库存

      大量

    • 是否单克隆

      1

    • 保存条件

      -20°c

    • 规格

      100 ul (10 western blots)/carrier free & custom formulation / quantity

    规格:产品价格:¥请询价
    规格:100 ul (10 western blots)产品价格:¥请询价
    规格:carrier free & custom formulation / quantity产品价格:¥请询价

    pathway more info application references datasheet PDF MSDS PDF protocols

    Applications Key:  W=Western Blotting  IP=Immunoprecipitation
    Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
    Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

    Applications Reactivity Sensitivity MW (kDa) Isotype
    W IP H M R Mk Endogenous 47 kDa Rabbit IgG
    Protocols
    Specificity / Sensitivity

    NCK1 (15B9) Rabbit mAb detects the endogenous levels of total NCK1 protein. Based on homology it may also recognize NCK2.

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly300 of human NCK1.

    Western Blotting

    Western Blotting

    Western blot analysis of total cell lysates from 293, A10, NIH/3T3 and COS cells, using NCK1 (15B9) Rabbit mAb.

    Background

    NCK1 (also known as NCK or NCKα) is a broadly expressed oncogenic adapter protein consisting of three SH3 domains and one SH2 domain (1-3). NCK1 becomes phosphorylated upon activation of variety of cell surface receptors and is involved in actin cytoskeletal organization induced by many stimuli (4-6). NCK2 (also known as NCKβ), a homolog of NCK1, has an overlapping expression pattern and redundant functions with NCK1 (7).

    1. Lehmann, J.M. et al. (1990) Nucleic Acids Res. 18, 1048.
    2. Chou, M.M. et al. (1992) Mol. Cell Biol. 12, 5834-5842.
    3. Li, W. et al. (1992) Mol. Cell Biol. 12, 5824-5833.
    4. Verma, R. et al. (2006) J. Clin. Invest. 116, 1346-1359.
    5. Rivera, G.M. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 9536-9541.
    6. Li, H. et al. (2006) Biochem. Biophys. Res. Commun. 349, 310-316.
    7. Bladt, F. et al. (2003) Mol. Cell Biol. 23, 4586-4597.
    Application References

    Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !

    Companion Products

    Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.


    For Research Use Only. Not For Use In Diagnostic Procedures.

    风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。

    图标文献和实验
    相关实验
    • Testing Rabbit Bleeds By Elisa

      same amount of NaBH4 after 15''. Do a quick microfuge spin if there are too many bubbles. B. ELISA with Peptide conjugated to BSA Coat 10 ug/ml antigen (peptide conjugated to BSA) diluted in TBS (50 ul/well) ON at 4 deg C. Remove antigen

    •  家兔(Rabbit,Oryctolagus curiculus)

      家兔(Rabbit,Oryctolagus curiculus)   一、生物学特性和解剖生理特点   1.家兔属于哺乳纲、啮齿目、兔科、草食性哺乳动性。   2.家兔体小力弱、胆小怕惊、怕热、怕潮,喜欢安静、清洁、干燥、凉爽的环境,不能忍受污秽的条件。   3.是食草类(Herbivorous)单胃动物。饲养原则是以青粗食米为主,精饲料为辅。喜欢独居,白天活动少,都处于假眠或休息状态,夜间活动大,吃食多。有啃木、扒土的习惯

    • Immunofluorescence: Rabbit Anti-Murine RELMα

      实验步骤   The following protocol used B6 mice that were infected with 200 embryonated Trichuris muris eggs and sacrificed 15 days post-infection. The tip of the cecum was removed, rinsed in 1X PBS pH 7.2, and fixed in 4% PFA

    图标技术资料

    暂无技术资料 索取技术资料

    同类产品报价

    产品名称
    产品价格
    公司名称
    报价日期
    询价
    美国CST中国公司(丁香通商铺)
    2023年10月16日询价
    ¥2972
    上海瑶韵生物科技有限公司
    2025年07月09日询价
    ¥2972
    北京智杰方远科技有限公司
    2025年06月18日询价
    询价
    上海邦景实业有限公司
    2025年06月25日询价
    询价
    上海谷研实业有限公司
    2025年07月09日询价
    NCK1 (15B9) Rabbit mAb
    询价