- 询价
- Cell Signaling Technology
- 2248
- USA
- 2025年08月15日
- W
- H,M,R
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
HER2/ErbB2 (44E7) Mouse mAb
- 抗原：
synthetic peptide corresponding to residues close to the carboxy-terminal sequence of human Her2/ErbB2
- 应用范围：
W
- 库存：
大量
- 适应物种：
H,M,R
- 保质期：
详见说明书
- 供应商：
CST
- 级别：
详见MSDS文件
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H (M) (R) | Endogenous | 185 | Mouse IgG1 |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | HER2/ErbB2 (44E7) Mouse mAb detects endogenous levels of Her2/ErbB2 in various cell lines. It does not cross-react with any other related proteins. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues close to the carboxy-terminal sequence of human Her2/ErbB2. Western Blotting
Western blot analysis of various cell lysates using HER2/ErbB2 (44E7) Mouse mAb. HER2/ErbB2 (44E7) Mouse mAb specifically recognizes Her2/ErbB2 in SK-BR-3, MDA-MB-453 and T47D cells, but does not cross-react with EGF receptors (ovexpressed in A431 cells), ErbB3 (overexpressed in Cos-7 cells), or ErbB4 (overexpressed in CEM cells). ( The CEM-ErbB4 cell lysate was provided by Dr. David Riese, Purdue University). |
| Background | The ErbB2 (HER2) proto-oncogene encodes a 185 kDa transmembrane, receptor-like glycoprotein with intrinsic tyrosine kinase activity (1). While ErbB2 lacks an identified ligand, ErbB2 kinase activity can be activated in the absence of a ligand when overexpressed and through heteromeric associations with other ErbB family members (2). Amplification of the ErbB2 gene and overexpression of its product are detected in almost 40% of human breast cancers (3). Binding of the c-Cbl ubiquitin ligase to ErbB2 at Tyr1112 leads to ErbB2 poly-ubiquitination and enhances degradation of this kinase (4). ErbB2 is a key therapeutic target in the treatment of breast cancer and other carcinomas and targeting the regulation of ErbB2 degradation by the c-Cbl-regulated proteolytic pathway is one potential therapeutic strategy. Phosphorylation of the kinase domain residue Tyr877 of ErbB2 (homologous to Tyr416 of pp60c-Src) may be involved in regulating ErbB2 biological activity. The major autophosphorylation sites in ErbB2 are Tyr1248 and Tyr1221/1222; phosphorylation of these sites couples ErbB2 to the Ras-Raf-MAP kinase signal transduction pathway (1,5). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
T-Cell Activation Using mAb to CD3
One of the most common ways to assess T cell activation is to measure T cell proliferation upon in vitro stimulation of T cells via antigen or agonistic antibodies to TCR. This protocol is written as a starting point for examining in vitro proliferation of mouse
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