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HER2/ErbB2 (44E7) Mouse mAb
synthetic peptide corresponding to residues close to the carboxy-terminal sequence of human Her2/ErbB2
W
大量
H,M,R
详见说明书
CST
详见MSDS文件
1
-20°c
100 ul (10 western blots)/carrier free & custom formulation / quantity
规格: | 产品价格: | ¥请询价 | |
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规格: | 100 ul (10 western blots) | 产品价格: | ¥请询价 |
规格: | carrier free & custom formulation / quantity | 产品价格: | ¥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
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W | H (M) (R) | Endogenous | 185 | Mouse IgG1 |
Protocols |
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Specificity / Sensitivity | HER2/ErbB2 (44E7) Mouse mAb detects endogenous levels of Her2/ErbB2 in various cell lines. It does not cross-react with any other related proteins. |
Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues close to the carboxy-terminal sequence of human Her2/ErbB2. Western BlottingWestern blot analysis of various cell lysates using HER2/ErbB2 (44E7) Mouse mAb. HER2/ErbB2 (44E7) Mouse mAb specifically recognizes Her2/ErbB2 in SK-BR-3, MDA-MB-453 and T47D cells, but does not cross-react with EGF receptors (ovexpressed in A431 cells), ErbB3 (overexpressed in Cos-7 cells), or ErbB4 (overexpressed in CEM cells). ( The CEM-ErbB4 cell lysate was provided by Dr. David Riese, Purdue University). |
Background | The ErbB2 (HER2) proto-oncogene encodes a 185 kDa transmembrane, receptor-like glycoprotein with intrinsic tyrosine kinase activity (1). While ErbB2 lacks an identified ligand, ErbB2 kinase activity can be activated in the absence of a ligand when overexpressed and through heteromeric associations with other ErbB family members (2). Amplification of the ErbB2 gene and overexpression of its product are detected in almost 40% of human breast cancers (3). Binding of the c-Cbl ubiquitin ligase to ErbB2 at Tyr1112 leads to ErbB2 poly-ubiquitination and enhances degradation of this kinase (4). ErbB2 is a key therapeutic target in the treatment of breast cancer and other carcinomas and targeting the regulation of ErbB2 degradation by the c-Cbl-regulated proteolytic pathway is one potential therapeutic strategy. Phosphorylation of the kinase domain residue Tyr877 of ErbB2 (homologous to Tyr416 of pp60c-Src) may be involved in regulating ErbB2 biological activity. The major autophosphorylation sites in ErbB2 are Tyr1248 and Tyr1221/1222; phosphorylation of these sites couples ErbB2 to the Ras-Raf-MAP kinase signal transduction pathway (1,5). |
Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know! |
Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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