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- Biotium
- 美国
- 90113
- 2025年09月06日
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- 详细信息
- 询价记录
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- 技术资料
- 保质期：
1年
- 英文名：
Aminooxy-biotin 90113
- 库存：
99
- 供应商：
Biotium
- CAS号：
1
- 保存条件：
室温或低温
Aminooxy (aka hydroxylamine or aminooxyacetamide) biotin readily reacts with aldehyde or ketone groups to form a stable oxime linkage under mild conditions, without the use of reducing agents.
- Stable labeling of aldehyde or ketone groups on polysaccharides, glycoproteins or antibodies.
- Mild labeling conditions, at neutral to acidic pH, in aqueous solutions.
- Label cell-surface sialic acid groups on glycans on living animal cells.
- White to off-white solid soluble in DMSO.
- MW: 515.55.
We also offer CF® Dye Aminooxy derivatives that are excellent options for fluorescent labeling. CF® dyes are Biotium’s line of next-generation fluorescent dyes that have improved brightness, photostability and water solubility compared to other fluorescent dyes.
Advantages of Aminooxy Labeling
Aminooxy groups react with molecules containing aldehyde or ketone groups to form a stable oxime linkage in aqueous solutions, at neutral/acidic pH. The reaction is fast and can be further accelerated using a catalyst like aniline. Aminooxy reagents are therefore superior to hydrazides, which also react with aldehydes or ketones but form unstable hydrazone linkages. Aminooxy reagents offer a convenient and rapid way to label glycoproteins with a detectable tag, such as a fluorescent dye or biotin, under mild conditions. The labeling process involves two steps, first introducing aldehyde groups into the glycoproteins by mild periodate oxidation, followed by treating the functionalized proteins with an aminooxy reagent. Aminooxy labeling of antibody glycosylation sites can be used as an alternative to succinimidyl ester labeling of amines, for antibodies where amine labeling affects the antibody binding affinity. Aminooxy biotin has also been used to efficiently label cell-surface sialic acid-containing glycans on living animal cells.
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文献和实验Biotin-4-fluorescein (B4F) is a convenient molecular probe for (strept)avidin and for unlabeled biotin in homogeneous fluorescence assays. The primary standard is a 16 μM working solution of d-biotin which is used to titrate an aliquot
【交流】DIG/Biotin标记的Southern/Northern杂交专题讨论
Southern印迹杂交（Southern blot）是1975年由英国人southern创建，是研究DNA图谱的基本技术，在遗传病诊断、DNA图谱分析及PCR产物分析等方面有重要价值。 早期的Southern印迹是将凝胶中的DNA变性后，经毛细管的虹吸作用，转移到硝酸纤维膜上。印迹方法如电转法、真空转移法；滤膜发展了尼龙膜、化学活化膜（如APT、ABM纤维素膜）等。利用Southern印迹法可进行克隆基因的酶切、图谱分析、基因组中某一基因的定性及定量分析、基因突变分析及限制性片断长度多态
Conjugation of Antibodies to Biotin
Antibodies can be conjugated to biotin by a number of chemical means. They can then be used in immunochemical procedures in conjunction with secondary reagents coupled to biotin-binding protein proteins such as avidin.
