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文献和实验Deuterium Labeling for Neutron Structure-Function-Dynamics Analysis
proteins can be readily produced by endogenous expression of recombinant proteins in bacterial systems that are adapted to growth in D2 O solution and using selectively deuterated carbon sources. Adaptation can be achieved either by gradual step-wise
Application of Amide Proton Exchange Mass Spectrometry for the Study of Protein‐Protein Interactions
of the fact that solvent deuterium oxide (D2 O) molecules are excluded from the surface of a protein to which another protein is bound. A more complete version of the experiment can also be performed in which the rate of surface deuteration is initially
(1) 分离的嗜碱性粒细胞,用Hanks液洗2次,每次1500r/min离心10mi; (2) 吸去上清液,留约0.3ml沉淀,轻轻振摇使细胞重悬; (3) 取过敏原50μl,与细胞悬液50μl混合,放置37℃水浴30min,以同量缓冲液代替过敏原作为对照管; (4) 加Alcian蓝染液150μl,混匀,置15~25℃过夜。次日计数9大方格内染成蓝色的嗜碱性粒细胞数(F); 【结果分
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