OxyBlot Protein Oxidation Dete

Immunohistochemical Detection of Protein Oxidation

, almost all oxidative modifications of proteins are resulting in an increase of carbonylated proteins. The three major pathways leading to carbonyl group formation (protein oxidation) are shown in Fig. 1 . Carbonyl groups are introduced into proteins as a result

Analysis of Protein Targets by Oxidative Stress Using the OxyBlot and BiotinAvidin-Capture Methodology

, two methodological approaches are presented which allow for the detection of protein carbonyl groups. While the OxyBlot methodology allows for the evaluation of general oxidative stress, the use of the novel and powerful biotin–avidin-capture

Diagonal Electrophoresis for Detection of Protein Disulphide Bridges

to oxidation, and this can result in transient formation of intra- or inter-chain disulphide bridges. Diagonal electrophoresis is a relatively simple technique to analyse the formation of these bridges by sequential non-reducing/reducing electrophoresis