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文献和实验网络 四、膜辅蛋白(CD46) 膜辅蛋白(membrane cofactor protein,MCP)由Cole等(1985)应用C3b亲和层析在外周血淋巴细胞上发现的一种膜蛋白。由于其奇特的电泳特征,起初被命名为gp45-70,但进一步研究发现,它对I因子介导的对C3b和C4b的裂解有辅助活性,故更命为MCP。鉴于MCP
Single Clone Excision Protocol
1.Pick plaques and put in 500 ml sterile SM with 20 ml chloroform.Store in 4° overnight. 2.Be sure we have SOLR and XL1-Blue cells in 10 mM MgSO4,no more than 1 week old.If not,set up a culture of each in LB supplemented with maltose and MgSO4 (add
Clone Genes From a Phage Library
). Its complexity is roughly 80,000 X an insert size of 4 to 8 kb = 320 Mbp, which is about 20-fold coverage of this yeast genome. The aim is to evenly plate out about 10,000 phage over about 10 plates. What you need in addition to the phage is: Plates: LB
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