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文献和实验Methylated CpG Island Amplification
hybridization solution (e.g. BLOTTO) Random-primed DNA labeling kit Wash solutions (Wash1 2xSSC, 0.1%SDS; Wash2 0.1XSSC, 0.1%SDS) 1.2. RDA and Cloning PCR Products 3 X EE buffer : 30 mM EPPS (SIGMA) pH 8.0, 3 mM
Detection of Anti-GOR Antibodies in HCV Infection
The interactions of the hepatitis C virus (HCV) with the immune system are numerous. As one of the many results, a considerable number of autoantibodies occur in serum. This chapter describes methods to detect anti-GOR antibodies (1 –
Methylated CpG Island Amplification (MCA)
Methylated CpG island Amplification Protocol written by Minoru Toyota 2. Materials 2.1. MCA Restriction enzymes SmaI, XmaI T4 DNA ligase Taq DNA polymerase 10X PCR reaction buffer: 670mM Tris-HCl, pH
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