- ￥1172
- Merck
- 美国
- AB5048P
- 2025年11月04日
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文献和实验How to Make Simple Solutions and Dilutions
= 200 ul = 0.2 ml C2 = the new concentration = 25 mg/ ml By algebraic rearrangement: V1 = (V2 x C2) / C1 V1 = (0.2 ml x 25 mg/ml) / 100 mg/ml and after cancelling the units, V1 = 0.05 ml, or 50 ul
Crystallization of Kinesin Family Motor Proteins
protein in 20 mM HEPES pH 7.5, 100 mM NaCl, 1 mM EGTA, 0.7 M Li2S04, 2 mM ADP, 10 mM MgCl2Reservoir: 20 mM HEPES pH 7.5, 1.4 M Li2SO4, 1 mM EGTA, 1 mM DTT, 10 mM MgCl2Sablin et al., 1998Ncd 295-700Hanging drop Room temperature5 mg/ml protein in 25 mM Na2PO
each wash. Add Rb IgG (4 ug) or anti-XKCM1 Gly (4 ug) and bring volume to 100 ul total. Bind antibody to beads at 4 deg.C for 1 hr 15' on rotator. Make sure beads are rolling around. Pellet in ufuge in coldroom and wash 1X TBST, 3X CSFXB
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