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- Merck
- 美国
- 539522-5MG
- 2025年07月12日
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文献和实验Electrophoretic Mobility Shift Assay Analysis of NFB Transcriptional Regulation by Nuclear IB
mobility shift assay (EMSA) to analyze the regulation of NFκB DNA binding by nuclear IκBα induced by the proteasome inhibitor MG132. Using this protocol, we show that in human leukemia Hut-78 cells that exhibit high levels of NFκB DNA binding activity, MG
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
:chloroform:isoamyl alcohol, UltraPure (25:24:1 v/v/v; Invitrogen) Phosphate-buffered saline (PBS; GIBCO 20012) Protease inhibitor cocktail (Sigma P8340) Proteinase K (10 mg/mL in TE buffer, pH 8.0; Invitrogen) PureLink HQ 96 Mini
, T12 MG, T12 MT ; where M is A,C or G) dilute DNA-free RNA to 0.1 µg/µl with H2 ODEPC , keep on ice set up cDNA synthesis reaction for each degenerate anchored oligo(dT) primer set: 4 µl 5 x reverse transcriptase buffer, 2 µl DTT
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