PROTEASE INHIB COCTAIL II 110U

CHIP染色质免疫共沉淀实验服务案例介绍

6.5 500 ul 10X Glycine 11 ml 10X PBS 24 ml Store at -20℃: Protease Inhibitor Cocktail II（蛋白酶抑制剂） 2×110 ul RNase

【求助】ChIP在超声后的电泳检测无DNA是怎么回事呢？

pellet in 1 mL of nuclei lysis buffer for ChIP supplemented with protease inhibitors and incubate for 10 min on ice. 8. Proceed with sonication. Ensure that the samples do not foam and are kept as cold as possible. 在完成1-8步骤后用 1. mix 20 ul

Immunoblot Protocol

1. Materials Protease inhibitor cocktail (Boehringer Mannheim) 2X SDS sample buffer (Novex) Pre-casted gels (e.g. Novex) Molecular weight protein standard (e.g. MultiMark Multi-Colored Standard, Novex) 25X Tris