2. Materials and methods
2.1. Chemicals and reagents
Emodin was purchased from Shanghai future industry Limited by Share Ltd
(Shanghai, China). BLM was purchased from Nippon Kayaku (Tokyo, Japan). TRIzol
Reagent was from Invitrogen Corporation (Carlsbad, USA). The ReverTra Ace qPCR
RT Kit and SYBR Green Real-time PCR Master Mix were purchased from TOYOBO(Osaka, Japan). Diff-quick staining kit was purchased from Zhuhai Baso
Bio-technology limited (Zhuhai, China). IL-6 and TNF-α ELISA kits were purchased
from Shanghai Weiao Biotech Ltd (Shanghai, China). Rabbit anti-α-SMA,
anti-HSP-47 and anti-collagen IV antibodies were purchased from Abcam
Biotechnology (Cambridge, MA, USA). Rabbit anti-p-Smad2, anti-p-Smad3,
anti-Smad2 and anti-Smad3 antibodies were purchased from Cell Signaling
Technology (Beverly, MA, USA). Rabbit anti-p-STAT3 antibody was purchased from
Absci (MD, USA). Rabbit anti-fibronectin and anti-STAT3 antibodies were
purchased from Proteintech (Chicago, IL, USA). Mouse anti-GAPDH polyclonal
antibody, HRP-labeled Goat Anti-Rabbit/Mouse IgG(H+L) and RIPA lysis buffer
were purchased from Beyotime Institute of Biotechnology (Haimen, China). The
poly-vinylidene fluoride (PVDF) membranes were from Millipore Corporation
(Billerica, MA, USA). ECL-Plus detection kit probed was from Tiangen Biotech Co.
Ltd (Beijing, China). The other chemicals and reagents used in the experiment were
of analytical grade.