Recombinant Human NKp80 (N-6Hi

Recombinant Human NKp80 (N-6His) (C15F)

产品说明(Description)

Recombinant Killer cell lectin-like receptor subfamily F member 1 is produced by our Mammalian expression system and the target gene encoding Val166-Tyr231 is expressed with a 6His tag at the N-terminus.

Accession #: Q9NZS2

Known as: Killer cell lectin-like receptor subfamily F member 1; Lectin-like receptor F1; KLRF1; Activating coreceptor NKp80; C-type lectin domain family 5 member C; CLEC5C

 

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

 

质量控制(Quality Control)

Purity:        Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin: Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.

 

复溶(Reconstitution)

 

Always centrifuge tubes before opening.  Do not mix by vortex or pipetting. 

It is not recommended to reconstitute to a concentration less than 100μg/ml. 

Dissolve the lyophilized protein in distilled water.

Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

 

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. 

Reconstituted protein solution can be stored at 4-7°C for 2-7 days.  

Aliquots of reconstituted samples are stable at < -20°C for 3 months.

 

背景(Background)

Killer cell lectin-like receptor subfamily F member 1 (KLRF1) is known as NKp80. Human NKp80, a member of the C-type lectin family of proteins (1 - 3), is 231 aa in length with a 38 amino acid (aa) cytoplasmic region, a 21 aa transmembrane region, and a 172 aa extracellular domain (ECD). The protein strongly expressed in peripheral blood leukocytes and spleen, with weaker expression in lymph node and adult liver, and no expression detected in bone marrow, thymus, and fetal liver. Its' function involved in the natural killer (NK)-mediated cytolysis of PHA-induced lymphoblasts.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY