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- 2025年12月05日
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FCH Group Screening Library
MCE 国际站：FCH Group Screening Library
FCH Group Screening Library Collection contains about 2,244,487 lead-like compounds for biological screening. This brand new collection comprises polar molecules with pharmacologically important groups such as free carboxylic and amino groups.
Description & Advantages:
• Provide more than 2,244,487 lead-like compounds.
• Polar molecules containing pharmacologically important groups, such as free carboxyl and amino groups.
• Diverse (chemical diversity > 0.8); Refined (PAINS-free, lead-like); Unique (> 99% exclusive compounds); Group-oriented (40% carboxylic acids, 27% primary amines, 30% secondary amines); Small, Shaped, and Soluble (average MW 315, average Fsp3 0.52, Sw > 30 μM for > 85%).
• Suitable for high throughput biological screening.
• Provides 3D optimized structure files for preparing virtual filters.
• Compounds can be provided in various forms: dry powder or solution placed in vials, microtubes or plates (96 or 384 wells).
• Databases and compounds are provided by FCH Group.
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文献和实验When trying to identify a clone within a cDNA library, which may contain a coat protein (CP) gene, one useful technique may by immunological screening, using antibodies raised against either purified virus or isolated CP. Antibody screening
: aliquot a portion of the stock to a separate tube and dilute to OD600 = 0.5-0.7 using 10mM MgSO4 PROCEDURE - Step 2 - Determine Library titer and Library plating 1. Grow ON culture of Y
Yeast Two-Hybrid Library Screening
The two-hybrid system was originally devised by Fields and Song as a protein interaction detection system in yeast (1 ). Subsequently, it has been employed in many laboratories as a means of screening cDNA and genomic fusion libraries
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