4-戊基苯基乙酸,2-(4-Pentylphenyl)ace

Separation of Histone Variants and Post‐Translationally Modified Isoforms by Triton/Acetic Acid/Urea Polyacrylamide Gel Electrophoresis

Due to their similarities in size and charge, complete resolution of histones by electrophoresis poses a considerable challenge. The addition of nonionic detergents to the traditional acetic acid/urea (AU) polyacrylamide gel electrophoresis (PAGE) system has afforded

Two dimensional peptide mapping

will probably go down. After exposure, cut out the membrane piece of interest and soak it immediately in 0.5% PVP-360 (Polyvinylpyrrolidone, MW360) in 100mM acetic acid for 30 min. at 37°C. If you don't do this, your yield will be poor. This may result

Characterization of Phosphopeptides Using a Combination of Immobilized Metal Ion Affinity Media and Direct Analysis by MALDI-TOF-MS

each with 30 µL of 100 mM acetic acid. To regenerate the columns with either Fe3+ or Ga3+ metal, wash the column three times each with 30 µL of either 60 mM GaCl3 or 100 mM FeCl3. To remove any unbound metal ions, wash the column three times each with 30 µL