Recombinant Human Cyclophilin

Recombinant Human Cyclophilin A (C-6His) (CR89)

产品说明(Description)

Recombinant Human Peptidyl-prolyl cis-trans isomerase A is produced by our E.coli expression system and the target gene encoding Val2-Glu165  is expressed with a 6His tag at the C-terminus.

Accession #: P62937

Known as: Peptidyl-prolyl cis-trans isomerase A; PPIA; PPIase A; Cyclosporin A-binding protein; Rotamase A; Cyclophilin A; Cyclosporin A-binding protein; CYPA

 

制剂(Formulation)

Supplied as a 0.2 μm filtered solution of PBS, 10% Glycerol, 1mM DTT, pH 7.4.

 

质量控制(Quality Control)

Purity:        Greater than 90% as determined by reducing SDS-PAGE.    

Endotoxin: Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.

 

 

运输(Shipping)

The product is shipped on dry ice/polar packs. 
Upon receipt, store it immediately at the temperature listed below.

 

保存(Storage)

Store at ≤-70°C, stable for 6 months after receipt.
Store at ≤-70°C, stable for 3 months under sterile conditions after opening. 
Please minimize freeze-thaw cycles.     

 

背景(Background)

Cyclophilin A, also known as peptidylprolyl isomerase A (PPIA), is an 18 kDa protein that catalyzes cis-trans isomerization at proline imidic peptide bonds, thereby promoting protein folding/trafficking and regulating protein activity. Cyclophilin A has multiple known functions in inflammation. Intracellularly, cyclophilin A interacts with interleukin (IL)-2 inducible T cell kinase (ITK) to tune T cell receptor signaling. Extracellularly, cyclophilin A is known to function as a leukocyte chemotactic factor. Cells secrete cyclophilin A by a vesicular secretory pathway in response to lipopolysaccharide and oxidative stress, or cyclophilin A may be released during cell death. Cyclophilin A influences inflammatory responses through its actions on immune activation and/or leukocyte trafficking.

 

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY