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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
咨询
- 保质期:
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- 英文名:
3-Bromo-5-fluorobenzene-1-sulfonyl chloride
- 库存:
咨询
- 供应商:
无锡云萃生物
- CAS号:
1214342-44-5
- 规格:
5mg/50mg/100mg/500mg
| 规格: | 5mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 50mg | 产品价格: | ¥100.0 |
| 规格: | 100mg | 产品价格: | ¥100.0 |
| 规格: | 500mg | 产品价格: | ¥100.0 |
云萃
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文献和实验, L broth and plates. -Hanahan (1983) J. Mol. Biol. 166, 557-580. HB101 - F[-], hsdS20 (rb[-], mb[-]), supE44, ara14, galK2, lacY1, proA2, rpsL20 (str[R]), xyl-5, mtl-1, [[lambda]][-], recA13, mcrA(+), mcrB(-)
20% Silver nitrate 20ml 95% Alcohol 20ml Add ammonia until precipitate formed is redissolved Add 10 more drops of ammonia 4. 5% Sodium thiosulphate 5. 0.1% Gold chloride METHOD Take sections to distilled
in cesium chloride-ethidium bromide gradients (1). Briefly, cells containing the desired plasmid or cosmid are harvested by centrifugation, incubated in a lysozyme buffer, and treated with alkaline detergent. Detergent solubilized proteins and membranes
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