1-碘庚烷,1-Iodoheptane,95% (stabi

Chromatin IP (CHIP assay)

7.5; 62.5 ml 4 M NaCl; 50 ml 10 % Triton X100; 5 ml 10 % NaDeoxycholate; protease inhibitors]  CHIP wash buffer: 10 mM Tris pH 8.0; 250 mM LiCl; 0.5 % NP-40; 0.5 % NaDeoxycholate; 1 mM EDTA. [ for 500 ml: 5 ml 1 M Tris pH 8.0; 5.3 g LiCl; 25 ml 10

ChIP-Chip E. coli

  Abstract ChIP-Chip stands for Ch romatin I mmunop recipitation and chip in the sense of DNA microarray. It is a technique to determine the genome-wide binding sites of a DNA-binding protein. While the basic principle

【求助】ChIP在超声后的电泳检测无DNA是怎么回事呢？

海之泪 有做过ChIP的高手不？ 我用冷泉港提供的protocol（http://cshprotocols.cshlp.org/cgi/content/full/2009/9/pdb.prot5279#R42） 1. Add 27 μL of 37% formaldehyde per milliliter of cell culture (in growth medium on tissue culture plates