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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
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- 英文名:
(S,S)-1,2-Bis(t-butylmethylphosphino)benzene (S,S)-BenzP
- 库存:
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- 供应商:
无锡云萃生物
- CAS号:
1355162-85-4
- 规格:
5mg/50mg/100mg/500mg
| 规格: | 5mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 50mg | 产品价格: | ¥100.0 |
| 规格: | 100mg | 产品价格: | ¥100.0 |
| 规格: | 500mg | 产品价格: | ¥100.0 |
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文献和实验Phosphoamino acid analysis:Mark Kamps's method
more than 3 µl is tedious,so don't use much H20.On the other hand,I wouldn't use much less than 6 µl so as to have good recovery. 7.Spot 1 µl PAA standards (about 0.3 µg each of PSer,PThr,and PTyr)on a cellulose thin layer chromatography plate and then spot
Co-staining with Thioflavin S & Carboxyfluorescein
Co-staining with Thioflavin S & Carboxyfluorescein 1.) Prepare 0.01% thioflavin S (T1892 , Sigma) solution in F12+ [0.01g / 100 ml F12+] 2.) Filter the staining solution with a 0.22 µm Millipore filter
).If the low plate has 100 colonies,your transformation efficiency was 102/0.01ng,which translates to 107/µg.I never had problems storing transformation control DNA at 0.1 ng/µl in the freezer,so you don’t have to dilute it each time you do a transformation
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