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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
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- 英文名:
6-Methoxypyridine-3-sulfonyl chloride
- 库存:
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- 供应商:
无锡云萃生物
- CAS号:
312300-42-8
- 规格:
5mg/50mg/100mg/500mg
| 规格: | 5mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 50mg | 产品价格: | ¥100.0 |
| 规格: | 100mg | 产品价格: | ¥100.0 |
| 规格: | 500mg | 产品价格: | ¥100.0 |
云萃
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文献和实验in cesium chloride-ethidium bromide gradients (1). Briefly, cells containing the desired plasmid or cosmid are harvested by centrifugation, incubated in a lysozyme buffer, and treated with alkaline detergent. Detergent solubilized proteins and membranes
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
20% Silver nitrate 20ml 95% Alcohol 20ml Add ammonia until precipitate formed is redissolved Add 10 more drops of ammonia 4. 5% Sodium thiosulphate 5. 0.1% Gold chloride METHOD Take sections to distilled
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