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文献和实验Detection of Cysteine S-Nitrosylation and Tyrosine 3-Nitration in Kidney Proteins
Nitric oxide (NO) is a diatomic free-radical product of mammalian cells that has diverse and important physiological functions, including the regulation of renal, cardiovascular, pulmonary, gastrointestinal, neuronal, inflammatory, and immune
Chan, W. 1968. A method for the complete S‐sulfonation of cysteine residues in proteins. Biochemistry. 12:4247‐4253. Chau, M.‐H. and Nelson, J.W. 1992
Fluorescent Labeling of Specific Cysteine Residues Using CyMPL
. However, it can be difficult to achieve specific labeling of a particular cysteine residue in a protein containing multiple cysteines, in a mixture of proteins, or in a protein's native environment. This unit describes a procedure termed CyMPL (Cysteine Metal Protection
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