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- BJ-01F13662
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Store at -20°C
- CAS号：
136156-13-3
- 规格：
50ug (solution)
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文献和实验E.Z.N.Z.TM MicroElute RNA Clean-up Protocol
upon addition of ethanol in Step 3. Vortex and add the entire mixture to the column. With the spin column inside a 2ml collection tube (supplied with kit), centrifuge at 10,000 x g for 30 seconds at room temperature. Discard flowthrough and re-use the collection
E.Z.N.Z.TM MicroElute RNA Desalting and Concentration protocol
(supplied with kit), centrifuge at 10,000 x g for 30 seconds at room temperature. Discard flowthrough and re-use the collection tube. 5. Place column in the same 2 ml collection tube from step 4 and add 500 ul RWB Wash Buffer diluted with absolute
Lac-Z Detection in Tail Biopsies
2mm tail biopsies are collected in 1X PBS (see recipes below) in a 1.5ml microfuge tube. Be sure to include a known +/+ (lac-Z negative) biopsy as a control. Replace 1X PBS solution over biopsy with 2.5% Glutaraldehyde diluted in SpM buffer
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