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文献和实验Neuroanatomical Tract-Tracing Using Biotinylated Dextran Amine
Biotinylated dextran amine (BDA) is a highly efficient and powerful marker for bidirectional tracing of nerve pathways in a wide variety of species at the light and electron microscopic level. The BDA tract-tracing method can readily
Blood-Brain Barrier Permeability Using Tracers and Immunohistochemistry
-dextran (MW: 62,000) Fluorescein isothiocynate-albumin (MW: 67,000) Horseradish peroxidase* (MW: 40,000)
2 EDTA800 mLType I water?/span> 20.5 mL10 N NaOH to pH 7.4x mLType I water1000 mLAutoclave or filter sterilize. SSPE can replace SSC plus NaPO4 , pH 6.7.50% Dextran sulfate MW 5000Dextran sulfate MW 5000 works just as well as the traditional MW 500
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