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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
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- 保质期:
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- 英文名:
4-Amino-3-nitropyridine;
- 库存:
咨询
- 供应商:
无锡云萃生物
- CAS号:
1681-37-4
- 规格:
10mg/50mg/100mg
| 规格: | 10mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 50mg | 产品价格: | ¥100.0 |
| 规格: | 100mg | 产品价格: | ¥100.0 |
云萃
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文献和实验Procedure for Transfection of Mammalian Cells
IMDM containing 20% fetal bovine serum, 1% glutamine, 1% aa In a six-well or 35 mm tissue culture plate, seed ~2x 105 cells per well in 2 ml IMDM containing 10% FBS and nonessential amino acids. Incubate the cells at 37E C
Replication timing by density transfer
C]- acetate or glucose 0.01% (w/v) [15 N]- ammonium sulfate Amino acids/supplements (as required, added to the same concentration as in normal synthetic medium) --dissolved in "-N" medium and filtered
RNase-Free DNase 30 Kunitz Units - - - GlutaMAX - 2mM - - Non-Essential Amino Acids - 1× - - HEPES - 10mM - - N2 - 1× - - B27 - 1× - - Heparin - 4μg/ml
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