- ￥2880
- Agrisera
- 瑞典
- AS12 2540
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海经科化学科技有限公司
- 抗体英文名：
Donkey anti-Mouse IgG (H&L), DyLight® 350 conjugated
- 规格：
1mg
| Immunogen |
| Purified Mouse IgG, whole molecule |
|
Host Donkey
|
|
Clonality Polyclonal
|
|
Purity Affinity purified donkey IgG
|
|
Format Lyophilized
|
|
Quantity 1 mg
|
|
Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
|
|
Storage Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.
|
| For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. |
| For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. |
|
Recommended dilution 1 : 20-1 : 2000 for most applications
|
名称：Donkey anti-Mouse IgG (H&L), DyLight® 350 conjugated
货号：AS12 2540
品牌：Agrisera
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文献和实验E L I S A w i t h p l a t e l e t s
for 1 hour with a 1 :1000 dilution of rat IgG2 a anti-mouse kappa chain monoclonal antibody conjugated to horse-radish peroxidase (LO-MK1 , LO/IMEX) reveal with o-phenylenediamine dihydrochloride (OPD) in revelation solution. SOLUTIONS
-purified goat anti-mouse IgG (Jackson Labs; 1 mg/ml stock used at 1:300 dilution) Texas Red-conjugated affinity-purified donkey anti-rabbit (Jackson Labs;1 mg/ml stock used at 1:200 dilution)
MHC I STREPTAMERS® 检测与分离抗原特异性 (CD8+ T) 细胞
浓度为 1x107cells/ 100 μl，从中取 1 - 2x106 cells； 2． 用去离子水将 10xBuffer IS 稀释为 1x，准备 3 ml 稀释后的 Buffer IS； 3．取 1ul Strep-Jactin®PE 或 Strep-Tactin®APC，0.8μl MHC I-Strep，与 1x Buffer IS 混匀为 10ul 的终体积，冰上孵育 45 分钟； 4．将上一步孵育完的 Streptamers 复合物加入到细胞悬液中轻轻吹打混匀，冰上孵育 45 分钟
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