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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海经科化学科技有限公司
- 抗体英文名:
KLK14 | Kallikrein 14
- 规格:
100ug
| Immunogen |
| KLH-conjugated synthetic peptides derived from N-terminal and C-terminal ends of human KLK14 Q9P0G3 |
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Host Rabbit
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Clonality Polyclonal
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Purity Affinity purified serum
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Format Lyophilized in PBS pH 7.4
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Quantity 100 µg
|
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Reconstitution For reconstitution add 100 µl of sterile water.
|
|
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
|
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Tested applications Immunohistochemistry (IHC), Western blot (WB)
|
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Recommended dilution 6 µg/ml (IHC), 1 : 1000 (WB)
|
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Expected | apparent MW
|
| 28 kDa |
名称:KLK14 | Kallikrein 14
货号:AS06 101
品牌:Agrisera
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文献和实验To date, prostate-specific antigen (PSA) has proven to be the most useful tumor marker for prostate cancer (1 –3 ). However, the PSA test does not discriminate well between men with benign disease and early prostate cancer
liteng1117 求助: 构建好了重组腺病毒进行蛋白表达,分子量是14kd,5%的浓缩胶70v,12%,或15%的分离胶110v,跑90min。湿转:100v,90min。封闭用5%的脱脂奶粉,封闭3h(因为背景深,以为封闭久会好些)。一抗1:3000,4度过夜,二抗1:5000,孵育1h,洗膜都是用TBST漂洗15min ×4次。但是显影后背景特别深,虽然可以看见目的条带,但还是有一些非特异性的条带出现,不知道是封闭出问题还是一抗浓度过高。
与激肽生成有关的蛋白酶。EC3. 4. 21. 8。广泛存在于血浆和组织中。可从狗的胰脏中提取出来,但从人、牛、马、狗、猫等的腮腺、颌下腺、舌下腺等也能获得同样的物质。虽不引起正常动物的血糖量的变化,但具有使切除胰脏的狗或人的糖尿病患者的血糖量降低的作用。它与胰岛素是不同的物质,口服也有效。
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