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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海经科化学科技有限公司
- 抗体英文名:
PsaA | PSI-A core protein of photosystem I
- 规格:
50ul
| Immunogen |
| N-terminal part of recombinant PsaA protein from Chlamydomonas reinhardtii P12154 |
|
Host Rabbit
|
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Clonality Polyclonal
|
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Purity Serum
|
|
Format Lyophilized
|
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Quantity 50 µl
|
|
Reconstitution For reconstitution add 50 µl of sterile water.
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|
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
|
|
Tested applications Immunogold (IG), Western blot (WB), Blue Native PAGE (BN-PAGE)
|
|
Recommended dilution 1 : 20 (IG), 1 : 1000-1 : 5000 (WB)
|
|
Expected | apparent MW
|
| 82 | 55-60 kDa |
名称:PsaA | PSI-A core protein of photosystem I
货号:AS06 172
品牌:Agrisera
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文献和实验This chapter contains the description of several methods used for the isolation of functional photosystem (PS)II core particles from wild-type (wt ), PSI-less, and CP47 histidine-tagged cells of the cyanobacterium Synechocystis sp. PCC 6803
and functional impairment, including release of pigments and/or dissociation of protein subunits. Moreover, it is difficult to isolate intact complexes from thylakoids containing mutated PSI that accumulate to lower levels. Hence, isolation of intact PSI core
Isolation of CP43 and CP47 Photosystem II Proximal Antenna Complexes From Plants
A single-column method to purify the CP43 and CP47 pigment-protein complexes of photosystem (PS)II from higher plants is presented. To validate the isolation procedure, three different species were used (Spinacea oleracea, Beta vulgaris
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