Donkey anti-Rabbit IgG (H&L),
TRITC conjugated, min. cross-reactivity to mouse IgG/serum- ¥3244
- Agrisera
- 瑞典
- AS10 1144
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 克隆性：
多克隆
- 供应商：
上海经科化学科技有限公司
- 抗体英文名：
Donkey anti-Rabbit IgG (H&L), TRITC conjugated, min. cross-reactivity to mouse IgG/serum
- 规格：
2mg
| Host Donkey |
|
Clonality Polyclonal
|
|
Purity Affinity purified IgG
|
|
Format Liquid
|
|
Quantity 2 mg
|
|
Storage Store at 2-8°C. Shelf life of this product is one year from date of receipt.
|
|
Recommended dilution The optimal working dilution should be determined by the investigator.
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名称：Donkey anti-Rabbit IgG (H&L), TRITC conjugated, min. cross-reactivity to mouse IgG/serum
货号：AS10 1144
品牌：Agrisera
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文献和实验, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
, 5% BSA, pH 7.4) for 2 h at room temperature and then probed for CTGF by incubation for 40 min with anti-CTGF antibody at 0.5 µg/ml in blocking buffer. After extensive washing at 37°C, the filters were incubated with either HRP-conjugated donkey anti
), GSK-3b (dilution1:1,000; 1 h at RT), or b-actin (dilution 1:10,000; 1 h at RT). 5. Wash the membrane three times for 5 min each in TBS-T. 6. Incubate the membrane for 1 h at RT with 30 mL of TBS-Tcontaining anti-mouse or anti-rabbit IgG
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