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- Agrisera
- 瑞典
- AS07 214
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海经科化学科技有限公司
- 抗体英文名：
RFA | Baker's yeast replication factor A
- 规格：
50ul
| Immunogen |
| RPA from Saccharomyces cerevisiae consisting of three subunits RFA1 (70 kDa), RFA2 (30 kDa) and RFA3 (14 kDa); overexpressed in E.coli and purified by chromatography; no affinity tags were added to any of three subunits |
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Host Rabbit
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Clonality Polyclonal
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Purity Serum
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Format Lyophilized
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Quantity 50 µl
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Reconstitution For reconstitution add 50 µl of sterile water
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Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Tested applications Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP), Western blot (WB)
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Recommended dilution ChIP, 1 : 20 000 (WB)
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Expected | apparent MW
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| 70 + 30 + 14 kDa |
名称：RFA | Baker's yeast replication factor A
货号：AS07 214
品牌：Agrisera
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文献和实验Replication timing by density transfer
in Ycomp+[12 C]-glucose+ alpha factor. [I don't particularly try to resuspend the cells in exactly the same volume they were in originally, but rather, in a volume that's convenient.] 4. Transfer to 37o C, wait for culture to reach 37o C
Replication timing by comparative hybridization
for normalization of the later samples). Do the quantitation for each gel and plot the mean values for each time point. Here's a typical plot showing replication of ARS1 and R14 (a fragment near the right end of chromosome
Replication timing using transient hemimethylation
of replication initiation in yeast chromosomes J. Cell Sci. Suppl. 19:51-58. Rationale of methylation timing approach The E. coli dam methylase adds methyl groups to adenine in the sequence 5' GATC 3' ( Marinus and Morris, 1973
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