- ￥3441
- 瑞典Agrisera
- 瑞典
- AS10 1152
- 2025年07月09日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
进口期货
- 供应商：
上海经科化学科技有限公司
- 抗体英文名：
Goat anti-Rat IgG (H&L), TRITC conjugated, min. cross-reactivity to human, mouse IgG
- 规格：
1mg
| Host Goat |
|
Clonality Polyclonal
|
|
Purity Affinity purified IgG
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|
Format Lyophilized
|
|
Quantity 1 mg
|
|
Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
|
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Storage Store lyophilized material at 2-8°C.
|
| For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. |
| For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. |
|
Recommended dilution 1 : 20-1 : 2000 (IHC), 1 : 50-1 : 5000 (ICC) (Flow cyt)
|
名称：Goat anti-Rat IgG (H&L), TRITC conjugated, min. cross-reactivity to human, mouse IgG
货号：AS10 1152
品牌：Agrisera
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文献和实验Immunohistochemistry using Anti-Ganglioside Antibodies
supernatant containing antiganglioside mAb overnight at 4°C. The time of incubation may range from 30 min. to overnight as determined by experience. 5. Wash with 1% BSA in PBS 4 times. 6. Incubate in FITC-conjugated goat anti-mouse IgM F(ab
Immunohistochemistry using Anti-Ganglioside Antibodies
supernatant containing antiganglioside mAb overnight at 4°C. The time of incubation may range from 30 min. to overnight as determined by experience. 5. Wash with 1% BSA in PBS 4 times. 6. Incubate in FITC-conjugated goat anti-mouse IgM F(ab
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
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