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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海经科化学科技有限公司
- 抗体英文名:
PRK ribulose-5-P-kinase | Phosphoribulokinase
- 规格:
50ug
| Immunogen |
| KLH-conjugated peptide derived from known PRK sequences including Arabidopsis thaliana UniProt: P25697, TAIR: At1g32060 |
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Host Rabbit
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Clonality Polyclonal
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Purity Affinity purified serum in PBS, pH 7.4
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Format Lyophilized
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Quantity 50 µg
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Reconstitution For reconstitution add 50 µl of sterile water.
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Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Tested applications Western blot (WB)
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Recommended dilution 1 : 1000 (WB)
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Expected | apparent MW
|
| 44 | 39 kDa (A. thaliana) |
名称:PRK ribulose-5-P-kinase | Phosphoribulokinase
货号:AS07 257
品牌:Agrisera
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文献和实验Cyclic AMP- Dependent Protein Kinase Activity Ratio Assay
of activation of cAMP-dependent protein kinase (cA-PrK) and subsequent protein phosphorylation. The involvement of cA-PrK in the response may be investigated by the use of the “cA-PrK activity ratio” assay, first introduced in 1973 (1 ).
is cDNA concentration 200 ng/ul too high for qRT-PCR-Rea
of sterile MQ water with the same result. What is this? -PRK- QUOTE(PRK @ Mar 9 2006, 11:37 AM) [snapback]43694[/snapback] The gene we are interested in looking at is not very abundant. At 100 ng/ul, the CT value is 30.9
Typical 5'-kinase labeling reactions included the DNA to be labeled, [[gamma]]-32-P-rATP, T4 polynucleotide kinase, and buffer (3). After incubation at 37degC, reactions are heat inactivated by incubation at 80degC. Portions
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