Donkey anti-Sheep IgG (H&L), D
yLight® 800 conjugated, min. cross-reactivity to human, mouse, or rabbit IgG- ￥3441
- Agrisera
- 瑞典
- AS12 2479
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海经科化学科技有限公司
- 抗体英文名：
Donkey anti-Sheep IgG (H&L), DyLight® 800 conjugated, min. cross-reactivity to human, mouse, or rabbit IgG
- 规格：
1mg
| Immunogen |
| Purified Sheep IgG, whole molecule |
|
Host Donkey
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Clonality Polyclonal
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Purity Affinity purified donkey IgG
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|
Format Lyophilized
|
|
Quantity 1 mg
|
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Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
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|
Storage Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.
|
| For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. |
| For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. |
|
Tested applications immunofluorescence (IF), IImmunohistochemisty (IHC), Western blot (WB)
|
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Recommended dilution 1 : 20-1 : 2000 for most applications
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名称：Donkey anti-Sheep IgG (H&L), DyLight® 800 conjugated, min. cross-reactivity to human, mouse, or rabbit IgG
货号：AS12 2479
品牌：Agrisera
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文献和实验E L I S A w i t h p l a t e l e t s
for 1 hour with a 1 :1000 dilution of rat IgG2 a anti-mouse kappa chain monoclonal antibody conjugated to horse-radish peroxidase (LO-MK1 , LO/IMEX) reveal with o-phenylenediamine dihydrochloride (OPD) in revelation solution. SOLUTIONS
-purified goat anti-mouse IgG (Jackson Labs; 1 mg/ml stock used at 1:300 dilution) Texas Red-conjugated affinity-purified donkey anti-rabbit (Jackson Labs;1 mg/ml stock used at 1:200 dilution)
at 1:200 dilution) Cy3-conjugated affinity-purified goat anti-mouse IgG (Jackson Labs; 1 mg/ml stock used at 1:300 dilution) Texas Red-conjugated affinity-purified donkey anti-rabbit (Jackson Labs;1 mg/ml stock used at 1:200 dilution)
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