- ￥23430
- Agrisera
- 瑞典
- AS11 1748
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海经科化学科技有限公司
- 抗体英文名：
ABA | Abscisic acid ELISA quantitation kit
- 规格：
1kit
| Background | This ELISA assay utilise the principle of competitive binding to measure the concentration of hormone in plant extracts. The ABA (abscisic acid C1) hormone and ABA-glucosylester specific antibodies are precoated to the surface of the reaction wells. The plant extract sample, containing an unknown amount of hormone, is mixed in the reaction well with a known amount of a tracer to react with a limited number of antibodies in the reaction wells. During incubation the hormone in the sample competes with the tracer for the antibody binding sites. Unbound hormone, tracer and plant extract are washed out of the reaction wells. Following substrate addition which reacts with a tracer bound to the antibody and produces a yellow-coloured product. The absorbance of the sample is converted to concentration of hormone by means of a standard curve which is produced by simultaneously treating standards along with the samples. |
| Reference | Bing-quin et al. (2019). Transcriptome profiling using RNA-seq to provide insights into foxtail millet seedling tolerance to short-term water deficit stress induced by PEG-6000. J. of Integrative Agriculture, DOI: 10.1016/S2095-3119(19)62576-1 . |
| Reaction wells | 5pcs for 480 assays, 60 strips with 8 wells |
| Tracer | 20 – 50 µl |
| Tracer diluent | 5xstock + 0.02 % NaN3 |
| Reaction and wash solution | 10xstock+0.02 % NaN3 |
| Stopping reagent | 2x stock, developed colour is stable for along period and stopping reagent needs to be used to prevent colour reaction from developing further |
| Substrate diluent | 10xstock +0 0.02 % NaN3 |
| Substrate | 100 mg |
| Standards | 600 µl of each: 15.6 pmol, 7.8 pmol, 3.9 pmol, 1.95 pmol, 975 fmol, 488fmol, 244 fmol, 122 fmol, 61 fmol, 30.5 fmol, 15.2 fmol |
| Assay development time | 4-5 hours |
| Sensitivity |0.01 to 10 pmol/50 µl or 4 pg/50 µl |
| Plant extract volume | 50 µl |
名称：ABA | Abscisic acid ELISA quantitation kit
货号：AS11 1748
品牌：Agrisera
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Quantifying Phytohormones in Transformed Plants
in the 220–300 nm range. Since UV spectroscopy is nondestructive, mass spectrometry and UV spectroscopy are complementary for the identification of cytokinins (9 ). Recently, qualitative LC-MS analysis has also been described for abscisic acid (ABA) (10
can provide a useful measurement of antigen or antibody concentration. One of the most useful of the immunoassays is the two-antibody "sandwich" ELISA. This assay is used to determine the antigen concentration in unknown samples. This ELISA is fast
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