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- 文献和实验
- 技术资料
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- 英文名:
N
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- 供应商:
无锡云萃生物
- CAS号:
40929-48-4
- 规格:
5mg/50mg/100mg/500mg
| 规格: | 5mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 50mg | 产品价格: | ¥100.0 |
| 规格: | 100mg | 产品价格: | ¥100.0 |
| 规格: | 500mg | 产品价格: | ¥100.0 |
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文献和实验in cesium chloride-ethidium bromide gradients (1). Briefly, cells containing the desired plasmid or cosmid are harvested by centrifugation, incubated in a lysozyme buffer, and treated with alkaline detergent. Detergent solubilized proteins and membranes
[3H] Choline Labeling and TNF Treatment of HL-60 Cells
] choline chloride is usually around 80 Ci/mmol. 5) Incubate cells with label for a period of 48 or 72 hours. --> If experiment requires more cells then incubate longer or else incubate only for 48 hrs. Be consistent in the labeling period
Choline Labeling and TNF Treatment of HL-60 Cells
cells/ml. 4) Add (0.5 µl/ml media) [3H] choline chloride to get ~0.5 µCi/ml. --> Specific activity of the [3H] choline chloride is usually around 80 Ci/mmol. 5) Incubate cells with label for a period of 48 or 72 hours.
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