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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
咨询
- 保质期:
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- 英文名:
N
- 库存:
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- 供应商:
无锡云萃生物
- CAS号:
135820-80-3
- 规格:
5mg/50mg/100mg/500mg
| 规格: | 5mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 50mg | 产品价格: | ¥100.0 |
| 规格: | 100mg | 产品价格: | ¥100.0 |
| 规格: | 500mg | 产品价格: | ¥100.0 |
云萃
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文献和实验Small Scale GST-Tag Purification Under Nature Conditions
, and keep at -80ºC enough cell pellet samples for optimization of small scale purification procedure and further scale-up. Once you set up the best purification conditions at low scale, you can scale-up the procedure. Example: 1) Grow 1L culture 2)
0.1M ATP (pH 7.2): 5.71% (w/w) ATP, 84.86% (w/w) H2 O, 9.43% (w/w) 2M Tris base. adjust to pH7.5 by 2M NaOH (~70-80µl); prepare about 700µl, dilute 2000 times (it is the final dilution in the spectrofotometric cell), check the optical density: C
it is necessary to heat to 60-80o C. to facilitate solubilization. Check pH. If it differs from neutral (~7.2-7.5) dramatically - adjust by diluted alkali / acid. Organic solvents. Phenol C6 H5 OH
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