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文献和实验Extraction of DNA using DNAzol® Reagent
the culture plate and gently pipet the lysate into an assay tube. Cell Pellets or Suspensions: Add 1 ml of DNAzol® Reagent to 1-3 × 107 cells, either in pellet or in suspension (volume 100 µl), pellet the cells and wash them with 0.9% NaCl. Pellet
6. Determine the basic cell shape of the bacteria. 7. Add the information on Gram stain and cell shape to the work done in Exercise 12.2. Exercise 12.4 - Prokaryote Cell Number by Dilution Plating LEVEL II Figure 12.5 Colony counter Materials
The MTT Cell Proliferation Assay
Cell number per well too high. Decrease cell density at plating.Contamination of culture with bacteria or yeast. Remedy Discard. View wells prior to addition of MTT Reagent to check for contamination. 4、Problem: Absorbance readings are too low
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